KC LAB PUBLICATIONS
2025
Halim CE, Deng S, Crasta KC, Yap CT. Interplay Between the Cytoskeleton and DNA Damage Response in Cancer Progression. Cancers 2025; 17 (8): 1378. https://doi.org/10.3390/cancers17081378
Antonino Glaviano, Samarendra K. Singh, E. Hui Clarissa Lee, Elena Okina, Hiu Yan Lam, Daniela Carbone, E. Premkumar Reddy, Mark J. O’Connor, Andrew Koff, Garima Singh, Justin Stebbing, Gautam Sethi, Karen Carmelina Crasta, Patrizia Diana, Khandan Keyomarsi, Michael B. Yaffe, Seth A. Wander, Aditya Bardia, Alan Prem Kumar, CELL CYCLE DYSREGULATION IN CANCER, Pharmacological Reviews 2025 77(2). https://doi.org/10.1016/j.pharmr.2024.100030
2024
Crasta KC, Faggioli F. Editorial for Special Issue “Cellular Senescence: Recent Cellular Advances and Discoveries”. Biomedicines. 2024; 12(12):2796. https://doi.org/10.3390/biomedicines12122796
Glaviano A, Wander SA, Baird RD, Yap KC, Lam HY, Toi M, Carbone D, Geoerger B, Serra V, Jones RH, Ngeow J, Toska E, Stebbing J, Crasta K, Finn RS, Diana P, Vuina K, de Bruin RAM, Surana U, Bardia A, Kumar AP. Mechanisms of sensitivity and resistance to CDK4/CDK6 inhibitors in hormone receptor-positive breast cancer treatment. Drug Resist Updat. 2024 Sep;76:101103. doi: 10.1016/j.drup.2024.101103.
Matius Robert, Rekha Jakhar, Bijin Veonice Au, Gracie Wee Ling Eng, Meng Wei, Alvin Kunyao Gao, You Heng Chuah, Karishma Sachaphibulkij, Isabelle Bonne, Kah Jing Lim, Indrajit Sinha, Daniel Boon Leng Teh, Lina Hsiu Kim Lim, Prashant Kumar, Navin Kumar Verma, Newman Siu Kwan Sze, Elaine Hsuen Lim, Derrick Sek Tong Ong, Jit Kong Cheong, Koji Itahana, John Edward Connolly, Karen Carmelina Crasta. Extracellular vesicles from therapy-induced senescent tumour cells distinctly suppress breast cancer via DKK1-mediated inflammatory response. Biorxv.
Robert M, Kennedy BK#, Crasta KC#. Therapy-induced senescence through the redox lens. Redox Biol. 2024 Jun 6;74:103228. doi: 10.1016/j.redox.2024.103228. Epub ahead of print. # Corresponding Authors.
Feng J, Chuah YH, Liang Y, Cipta NO, Zeng Y, Warrier T, Elfar GARE, Yoon J, Grinchuk OV, Tay EXY, Lok KZ, Zheng ZQ, Khong ZJ, Chong ZS, Teo J, Sanford EM, Neo CJY, Chiu HY, Leung JY, Wang LC, Lim YT, Zhao T, Sobota RM, Crasta KC, Tergaonkar V, Taneja R, Ng SY, Cheok CF, Ling SC, Loh YH, Ong DST. PHF2 regulates genome topology and DNA replication in neural stem cells via cohesin. Nucleic Acids Res. 2024 May 29:gkae457. doi: 10.1093/nar/gkae457.
Guan L, Eisenmenger A, Crasta KC, Sandalova E, Maier AB. Therapeutic effect of dietary ingredients on cellular senescence in animals and humans: A systematic review. Ageing Res Rev. 2024 Mar;95:102238. doi: 10.1016/j.arr.2024.102238.
2023
Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST. CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma. Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3.
Elazary Y, Cheow K, Cheng RK, Ghosh R, Shainer I, Wexler Y, Crasta K, Gothilf Y, Jesuthasan SJ. Glial cells expressing visual cycle genes are vital for photoreceptor survival in the zebrafish pineal gland. J Pineal Res. 2023 Apr;74(3):e12854. doi: 10.1111/jpi.12854.
2022
Guan L, Crasta KC, Maier AB. Assessment of cell cycle regulators in human peripheral blood cells as markers of cellular senescence. Ageing Res Rev. 2022 Jun;78:101634. doi: 10.1016/j.arr.2022.101634.
Srinivas US, Tay NSC, Jaynes P, Anbuselvan A, Ramachandran GK, Wardyn JD, Hoppe MM, Hoang PM, Peng Y, Lim S, Lee MY, Peethala PC, An O, Shendre A, Tan BWQ, Jemimah S, Lakshmanan M, Hu L, Jakhar R, Sachaphibulkij K, Lim LHK, Pervaiz S, Crasta K, Yang H, Tan P, Liang C, Ho L, Khanchandani V, Kappei D, Yong WP, Tan DSP, Bordi M, Campello S, Tam WL, Frezza C, Jeyasekharan AD. PLK1 inhibition selectively induces apoptosis in ARID1A deficient cells through uncoupling of oxygen consumption from ATP production. Oncogene. 2022 Mar;41(13):1986-2002. doi: 10.1038/s41388-022-02219-8.
Yoon J, Grinchuk OV, Tirado-Magallanes R, Ngian ZK, Tay EXY, Chuah YH, Lee BWL, Feng J, Crasta KC, Ong CT, Benoukraf T, Ong DST. E2F and STAT3 provide transcriptional synergy for histone variant H2AZ activation to sustain glioblastoma chromatin accessibility and tumorigenicity. Cell Death Differ. 2022 Jul;29(7):1379-1394. doi: 10.1038/s41418-021-00926-5.
Robert M, Crasta K. Breaking the vicious circle: Extrachromosomal circular DNA as an emerging player in tumour evolution. Semin Cell Dev Biol. 2022 Mar;123:140-150. doi: 10.1016/j.semcdb.2021.11.015.
Crasta K. Chromothripsis: A shattered chromosome in the spotlight (Editorial). Semin Cell Dev Biol. 2022 Mar;123:88-89. doi: 10.1016/j.semcdb.2021.11.021.
+ All publications above are outcomes of work started at NUS.
Halim CE, Deng S, Crasta KC, Yap CT. Interplay Between the Cytoskeleton and DNA Damage Response in Cancer Progression. Cancers 2025; 17 (8): 1378. https://doi.org/10.3390/cancers17081378
Antonino Glaviano, Samarendra K. Singh, E. Hui Clarissa Lee, Elena Okina, Hiu Yan Lam, Daniela Carbone, E. Premkumar Reddy, Mark J. O’Connor, Andrew Koff, Garima Singh, Justin Stebbing, Gautam Sethi, Karen Carmelina Crasta, Patrizia Diana, Khandan Keyomarsi, Michael B. Yaffe, Seth A. Wander, Aditya Bardia, Alan Prem Kumar, CELL CYCLE DYSREGULATION IN CANCER, Pharmacological Reviews 2025 77(2). https://doi.org/10.1016/j.pharmr.2024.100030
2024
Crasta KC, Faggioli F. Editorial for Special Issue “Cellular Senescence: Recent Cellular Advances and Discoveries”. Biomedicines. 2024; 12(12):2796. https://doi.org/10.3390/biomedicines12122796
Glaviano A, Wander SA, Baird RD, Yap KC, Lam HY, Toi M, Carbone D, Geoerger B, Serra V, Jones RH, Ngeow J, Toska E, Stebbing J, Crasta K, Finn RS, Diana P, Vuina K, de Bruin RAM, Surana U, Bardia A, Kumar AP. Mechanisms of sensitivity and resistance to CDK4/CDK6 inhibitors in hormone receptor-positive breast cancer treatment. Drug Resist Updat. 2024 Sep;76:101103. doi: 10.1016/j.drup.2024.101103.
Matius Robert, Rekha Jakhar, Bijin Veonice Au, Gracie Wee Ling Eng, Meng Wei, Alvin Kunyao Gao, You Heng Chuah, Karishma Sachaphibulkij, Isabelle Bonne, Kah Jing Lim, Indrajit Sinha, Daniel Boon Leng Teh, Lina Hsiu Kim Lim, Prashant Kumar, Navin Kumar Verma, Newman Siu Kwan Sze, Elaine Hsuen Lim, Derrick Sek Tong Ong, Jit Kong Cheong, Koji Itahana, John Edward Connolly, Karen Carmelina Crasta. Extracellular vesicles from therapy-induced senescent tumour cells distinctly suppress breast cancer via DKK1-mediated inflammatory response. Biorxv.
Robert M, Kennedy BK#, Crasta KC#. Therapy-induced senescence through the redox lens. Redox Biol. 2024 Jun 6;74:103228. doi: 10.1016/j.redox.2024.103228. Epub ahead of print. # Corresponding Authors.
Feng J, Chuah YH, Liang Y, Cipta NO, Zeng Y, Warrier T, Elfar GARE, Yoon J, Grinchuk OV, Tay EXY, Lok KZ, Zheng ZQ, Khong ZJ, Chong ZS, Teo J, Sanford EM, Neo CJY, Chiu HY, Leung JY, Wang LC, Lim YT, Zhao T, Sobota RM, Crasta KC, Tergaonkar V, Taneja R, Ng SY, Cheok CF, Ling SC, Loh YH, Ong DST. PHF2 regulates genome topology and DNA replication in neural stem cells via cohesin. Nucleic Acids Res. 2024 May 29:gkae457. doi: 10.1093/nar/gkae457.
Guan L, Eisenmenger A, Crasta KC, Sandalova E, Maier AB. Therapeutic effect of dietary ingredients on cellular senescence in animals and humans: A systematic review. Ageing Res Rev. 2024 Mar;95:102238. doi: 10.1016/j.arr.2024.102238.
2023
Chuah YH, Tay EXY, Grinchuk OV, Yoon J, Feng J, Kannan S, Robert M, Jakhar R, Liang Y, Lee BWL, Wang LC, Lim YT, Zhao T, Sobota RM, Lu G, Low BC, Crasta KC, Verma CS, Lin Z, Ong DST. CAMK2D serves as a molecular scaffold for RNF8-MAD2 complex to induce mitotic checkpoint in glioma. Cell Death Differ. 2023 Aug;30(8):1973-1987. doi: 10.1038/s41418-023-01192-3.
Elazary Y, Cheow K, Cheng RK, Ghosh R, Shainer I, Wexler Y, Crasta K, Gothilf Y, Jesuthasan SJ. Glial cells expressing visual cycle genes are vital for photoreceptor survival in the zebrafish pineal gland. J Pineal Res. 2023 Apr;74(3):e12854. doi: 10.1111/jpi.12854.
2022
Guan L, Crasta KC, Maier AB. Assessment of cell cycle regulators in human peripheral blood cells as markers of cellular senescence. Ageing Res Rev. 2022 Jun;78:101634. doi: 10.1016/j.arr.2022.101634.
Srinivas US, Tay NSC, Jaynes P, Anbuselvan A, Ramachandran GK, Wardyn JD, Hoppe MM, Hoang PM, Peng Y, Lim S, Lee MY, Peethala PC, An O, Shendre A, Tan BWQ, Jemimah S, Lakshmanan M, Hu L, Jakhar R, Sachaphibulkij K, Lim LHK, Pervaiz S, Crasta K, Yang H, Tan P, Liang C, Ho L, Khanchandani V, Kappei D, Yong WP, Tan DSP, Bordi M, Campello S, Tam WL, Frezza C, Jeyasekharan AD. PLK1 inhibition selectively induces apoptosis in ARID1A deficient cells through uncoupling of oxygen consumption from ATP production. Oncogene. 2022 Mar;41(13):1986-2002. doi: 10.1038/s41388-022-02219-8.
Yoon J, Grinchuk OV, Tirado-Magallanes R, Ngian ZK, Tay EXY, Chuah YH, Lee BWL, Feng J, Crasta KC, Ong CT, Benoukraf T, Ong DST. E2F and STAT3 provide transcriptional synergy for histone variant H2AZ activation to sustain glioblastoma chromatin accessibility and tumorigenicity. Cell Death Differ. 2022 Jul;29(7):1379-1394. doi: 10.1038/s41418-021-00926-5.
Robert M, Crasta K. Breaking the vicious circle: Extrachromosomal circular DNA as an emerging player in tumour evolution. Semin Cell Dev Biol. 2022 Mar;123:140-150. doi: 10.1016/j.semcdb.2021.11.015.
Crasta K. Chromothripsis: A shattered chromosome in the spotlight (Editorial). Semin Cell Dev Biol. 2022 Mar;123:88-89. doi: 10.1016/j.semcdb.2021.11.021.
+ All publications above are outcomes of work started at NUS.
KC LAB MOVED TO THE NATIONAL UNIVERSITY OF SINGAPORE (NUS) IN DEC 2020.
YIKES - COVID HAPPENED!! AND OUR LAB THAT WAS ALL SET TO MOVE FROM NTU TO NUS IN APRIL 2020 WAS IN LIMBO.
YIKES - COVID HAPPENED!! AND OUR LAB THAT WAS ALL SET TO MOVE FROM NTU TO NUS IN APRIL 2020 WAS IN LIMBO.
Ku JWK, Chen Y, Lim BJW, Gasser S, Crasta KC, Gan YH. Bacterial-induced cell fusion is a danger signal triggering cGAS-STING pathway via micronuclei formation. Proc Natl Acad Sci U S A. 2020 Jul 7;117(27):15923-15934. doi: 10.1073/pnas.2006908117.
Tay VSY, Devaraj S, Koh T, Ke G, Crasta KC, Ali Y. Increased double strand breaks in diabetic β-cells with a p21 response that limits apoptosis. Sci Rep. 2019 Dec 18;9(1):19341. doi: 10.1038/s41598-019-54554-8.
Jakhar R, Crasta K. Exosomes as Emerging Pro-Tumorigenic Mediators of the Senescence-Associated Secretory Phenotype. Int J Mol Sci. 2019 May 24;20(10):2547. doi: 10.3390/ijms20102547.
Wong A, Chen S, Yang LK, Kanagasundaram Y, Crasta K. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov. 2018 Nov 27;4:109. doi: 10.1038/s41420-018-0127-5.
Jakhar R, Luijten MNH, Wong AXF, Cheng B, Guo K, Neo SP, Au B, Kulkarni M, Lim KJ, Maimaiti J, Chong HC, Lim EH, Tan TBK, Ong KW, Sim Y, Wong JSL, Khoo JBK, Ho JTS, Chua BT, Sinha I, Wang X, Connolly JE, Gunaratne J, Crasta KC. Autophagy Governs Protumorigenic Effects of Mitotic Slippage-induced Senescence. Mol Cancer Res. 2018 Nov;16(11):1625-1640. doi: 10.1158/1541-7786.MCR-18-0024.
This article was featured in "Highlights of This Issue":
Highlights of This Issue | Molecular Cancer Research | American Association for Cancer Research (aacrjournals.org)
Shyu P Jr*, Wong XFA*, Crasta K#, Thibault G#. Dropping in on lipid droplets: insights into cellular stress and cancer. Biosci Rep. 2018 Sep 19;38(5):BSR20180764. doi: 10.1042/BSR20180764.
He Q, Au B, Kulkarni M, Shen Y, Lim KJ, Maimaiti J, Wong CK, Luijten MNH, Chong HC, Lim EH, Rancati G, Sinha I, Fu Z, Wang X, Connolly JE, Crasta KC. Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects. Oncogenesis. 2018 Aug 15;7(8):62. doi: 10.1038/s41389-018-0072-4.
Luijten MNH, Lee JXT, Crasta KC. Mutational game changer: Chromothripsis and its emerging relevance to cancer. Mutat Res Rev Mutat Res. 2018 Jul-Sep;777:29-51. doi: 10.1016/j.mrrev.2018.06.004.
Luijten MNH, Lee JXT, Chen S, Crasta KC. Generation of Micronuclei and Detection of Chromosome Pulverization. Methods Mol Biol. 2018;1769:183-195. doi: 10.1007/978-1-4939-7780-2_12.
Crasta K, Aneja R. 50 years on … the discovery of tubulin continues to advance cancer treatment. Endocr Relat Cancer. 2017 Sep;24(9):E3-E5. doi: 10.1530/ERC-17-0273. Editorial-Special Issue on "50 years of discovery of Tubulin".
Cheng B, Crasta K. Consequences of mitotic slippage for antimicrotubule drug therapy. Endocr Relat Cancer. 2017 Sep;24(9):T97-T106. doi: 10.1530/ERC-17-0147.
B Cheng, K Crasta (2017). Autophagy, Cellular Senescence and Cancer. Chapter 4 pp 67-90. in Autophagy and Signaling. CRC Press, Taylor and Francis Group. https://doi.org/10.1201/9781315120638.
Tay VSY, Devaraj S, Koh T, Ke G, Crasta KC, Ali Y. Increased double strand breaks in diabetic β-cells with a p21 response that limits apoptosis. Sci Rep. 2019 Dec 18;9(1):19341. doi: 10.1038/s41598-019-54554-8.
Jakhar R, Crasta K. Exosomes as Emerging Pro-Tumorigenic Mediators of the Senescence-Associated Secretory Phenotype. Int J Mol Sci. 2019 May 24;20(10):2547. doi: 10.3390/ijms20102547.
Wong A, Chen S, Yang LK, Kanagasundaram Y, Crasta K. Lipid accumulation facilitates mitotic slippage-induced adaptation to anti-mitotic drug treatment. Cell Death Discov. 2018 Nov 27;4:109. doi: 10.1038/s41420-018-0127-5.
Jakhar R, Luijten MNH, Wong AXF, Cheng B, Guo K, Neo SP, Au B, Kulkarni M, Lim KJ, Maimaiti J, Chong HC, Lim EH, Tan TBK, Ong KW, Sim Y, Wong JSL, Khoo JBK, Ho JTS, Chua BT, Sinha I, Wang X, Connolly JE, Gunaratne J, Crasta KC. Autophagy Governs Protumorigenic Effects of Mitotic Slippage-induced Senescence. Mol Cancer Res. 2018 Nov;16(11):1625-1640. doi: 10.1158/1541-7786.MCR-18-0024.
This article was featured in "Highlights of This Issue":
Highlights of This Issue | Molecular Cancer Research | American Association for Cancer Research (aacrjournals.org)
Shyu P Jr*, Wong XFA*, Crasta K#, Thibault G#. Dropping in on lipid droplets: insights into cellular stress and cancer. Biosci Rep. 2018 Sep 19;38(5):BSR20180764. doi: 10.1042/BSR20180764.
He Q, Au B, Kulkarni M, Shen Y, Lim KJ, Maimaiti J, Wong CK, Luijten MNH, Chong HC, Lim EH, Rancati G, Sinha I, Fu Z, Wang X, Connolly JE, Crasta KC. Chromosomal instability-induced senescence potentiates cell non-autonomous tumourigenic effects. Oncogenesis. 2018 Aug 15;7(8):62. doi: 10.1038/s41389-018-0072-4.
Luijten MNH, Lee JXT, Crasta KC. Mutational game changer: Chromothripsis and its emerging relevance to cancer. Mutat Res Rev Mutat Res. 2018 Jul-Sep;777:29-51. doi: 10.1016/j.mrrev.2018.06.004.
Luijten MNH, Lee JXT, Chen S, Crasta KC. Generation of Micronuclei and Detection of Chromosome Pulverization. Methods Mol Biol. 2018;1769:183-195. doi: 10.1007/978-1-4939-7780-2_12.
Crasta K, Aneja R. 50 years on … the discovery of tubulin continues to advance cancer treatment. Endocr Relat Cancer. 2017 Sep;24(9):E3-E5. doi: 10.1530/ERC-17-0273. Editorial-Special Issue on "50 years of discovery of Tubulin".
Cheng B, Crasta K. Consequences of mitotic slippage for antimicrotubule drug therapy. Endocr Relat Cancer. 2017 Sep;24(9):T97-T106. doi: 10.1530/ERC-17-0147.
B Cheng, K Crasta (2017). Autophagy, Cellular Senescence and Cancer. Chapter 4 pp 67-90. in Autophagy and Signaling. CRC Press, Taylor and Francis Group. https://doi.org/10.1201/9781315120638.
Early Exposure in Mitotic Regulation and Cancer Biology:
2012 Crasta K., Ganem N., Dagher R., Lantermann A., Ivanova E., Potopova A., Yunfeng P., Nezi L., Chowdhury D and
Pellman D. DNA breaks and chromosome pulverization from errors in mitosis. Nature 482: 53-58.
2008 Crasta K., Lim H. H., Giddings,T.J. ,Winey M. and Surana U. Inactivation of Cdh1 by synergistic action of Cdk1 and
Polo kinase is necessary for proper assembly of a bipolar spindle. Nat Cell Biol 10: 665-675.
Crasta K., Lim H.H, Zhang T., Nirantar S. and Surana U. Consorting kinases, end of destruction and birth of a spindle.
Cell Cycle 7: 2960-2966.
2006 Crasta K., Huang P., Morgan G., Winey M. and Surana, U. Cdk1 regulates centrosome separation by restraining
proteolysis of microtubule-associated proteins. EMBO J. 25: 2551-2563.
Crasta K. and Surana U. Disjunction of conjoined twins: Cdk1, Cdh1 and separation of centrosomes. Cell Div 1: 12-14.
2004 Krishnan V., Nirantar S., Crasta K., Cheng A.Y.H. and Surana U. DNA replication checkpoint prevents
precocious chromosome segregation by regulating spindle behavior. Mol Cell 16: 687-700.
My publication as a NUS undergraduate on a study that sealed my love for scientific discovery .....
2002 Crasta K.C., Chua K.L., Subramaniam S., Frey J., Loh H. and Tan H.M. Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer. J Bacteriol 184(7): 1932-1939.
2012 Crasta K., Ganem N., Dagher R., Lantermann A., Ivanova E., Potopova A., Yunfeng P., Nezi L., Chowdhury D and
Pellman D. DNA breaks and chromosome pulverization from errors in mitosis. Nature 482: 53-58.
2008 Crasta K., Lim H. H., Giddings,T.J. ,Winey M. and Surana U. Inactivation of Cdh1 by synergistic action of Cdk1 and
Polo kinase is necessary for proper assembly of a bipolar spindle. Nat Cell Biol 10: 665-675.
Crasta K., Lim H.H, Zhang T., Nirantar S. and Surana U. Consorting kinases, end of destruction and birth of a spindle.
Cell Cycle 7: 2960-2966.
2006 Crasta K., Huang P., Morgan G., Winey M. and Surana, U. Cdk1 regulates centrosome separation by restraining
proteolysis of microtubule-associated proteins. EMBO J. 25: 2551-2563.
Crasta K. and Surana U. Disjunction of conjoined twins: Cdk1, Cdh1 and separation of centrosomes. Cell Div 1: 12-14.
2004 Krishnan V., Nirantar S., Crasta K., Cheng A.Y.H. and Surana U. DNA replication checkpoint prevents
precocious chromosome segregation by regulating spindle behavior. Mol Cell 16: 687-700.
My publication as a NUS undergraduate on a study that sealed my love for scientific discovery .....
2002 Crasta K.C., Chua K.L., Subramaniam S., Frey J., Loh H. and Tan H.M. Identification and Characterization of CAMP Cohemolysin as a Potential Virulence Factor of Riemerella anatipestifer. J Bacteriol 184(7): 1932-1939.